Association of VDR gene polymorphism (rs2228570 A>C, G, T) with risk of Benign Prostatic Hyperplasia in Iraqi patients

Abstract

Benign prostatic hyperplasia (BPH) represents a prevalent urological condition in aging men, characterized by prostate enlargement as well as lower urinary tract symptoms. We investigated the link between the VDR gene variant rs2228570 (A>C, G, T) and the risk for BPH in Iraqi patients. A total of 3 patients with BPH, as well as 10 healthy individuals (50–70 years) recruited in Baqubah Teaching Hospital, Diyala, participated in this investigation. The genomic DNA was extracted and amplified by PCR, and then sequenced by Geneious software. Three genotypes (AA, AG, GG) were found. We found a significant association between genotype distribution and risk of BPH. The GG genotype and G allele were more common in the controls with a significant protective effect (P = 0.001; OR = 29.00). Conversely, the AA genotype and A allele were common in the patients with a significant risk factor (P = 0.026). No significant effect was identified for the AG genotype (P = 0.862). Hardy–Weinberg testing revealed equilibrium for both populations without any evidence for genetic instability. In sum, these findings indicate that the VDR gene rs2228570 can be involved in the susceptibility towards BPH, underscoring its value in terms of testing genetic risk as well as preventing its occurrences